Proteintech, 66579-1-Ig-150ul, AMPK Beta 2 Monoclonal antibody

66579-1-Ig targets AMPK Beta 2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications
Positive WB detected in: A375 cells, A431 cells, HeLa cells, A549 cells, human brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected in: HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:1000-1:3000
Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
Background Information
AMPK beta 2 is a regulatory subunit of the AMP-activated protein kinase (AMPK), which is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK beta 2 may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.