Proteintech, 66620-1-Ig-20ul, ADAM10 Monoclonal antibody

66620-1-Ig targets ADAM10 in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: THP-1 cells, MCF-7 cells, Jurkat cells, K-562 cells
Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:1000-1:6000
Immunohistochemistry (IHC): IHC : 1:1000-1:4000
Background Information
ADAM10, a member of the ADAM (a disintegrin and metalloprotease) family, is widely expressed in the brain, the spinal cord, and the visual system during development.MW of premature ADAM10 is 90 kDa and mature ADAM10 is 60-70 kDa (PMID: 24404179).