Proteintech, 66747-1-Ig-20ul, androgen receptor Monoclonal antibody

66747-1-Ig targets androgen receptor in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: LNCaP cells, human testis tissue, NCCIT cells
Positive IHC detected in: human prostate cancer tissue, mouse testis tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: human prostate cancer tissue
Positive IF/ICC detected in: LNCaP cells
Recommended dilution
Western Blot (WB): WB : 1:600-1:3000
Immunohistochemistry (IHC): IHC : 1:5000-1:20000
Immunofluorescence (IF)-P: IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Background Information
AR, also named as DHTR and NR3C4, belongs to the nuclear hormone receptor family and NR3 subfamily. AR is a ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. AR is activated, but not phosphorylated, by HIPK3. Defects in AR are the cause of androgen insensitivity syndrome (AIS), previously known as testicular feminization syndrome (TFM), which is an X-linked recessive form of pseudohermaphroditism due end-organ resistance to androgen. Defects in AR are the cause of spinal and bulbar muscular atrophy X-linked type 1 (SMAX1) which also known as Kennedy disease. Defects in AR may play a role in metastatic prostate cancer. Defects in AR are the cause of androgen insensitivity syndrome partial (PAIS) which also known as Reifenstein syndrome. AR exists various isoforms with MW 110-120 kDa and 75-80 kDa. (PMID: 19244107 )