Proteintech, 66768-1-Ig-150ul, AGR2 Monoclonal antibody

66768-1-Ig targets AGR2 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, pig samples.
Tested Applications
Positive WB detected in: pig stomach tissue, T-47D cells, HT-29 cells
Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: human colon cancer tissue
Positive IF/ICC detected in: HT-29 cells, A549 cells
Positive FC (Intra) detected in: HT-29 cells
Recommended dilution
Western Blot (WB): WB : 1:1000-1:6000
Immunohistochemistry (IHC): IHC : 1:150-1:600
Immunofluorescence (IF)-P: IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Background Information
AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used to aide in assessment of high-risk patients undergoing endoscopic procedures.