Proteintech, 67715-1-Ig-150ul, Alix Monoclonal antibody

67715-1-Ig targets Alix in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: HSC-T6 cells, A431 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:2000-1:20000
Immunohistochemistry (IHC): IHC : 1:4000-1:16000
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Background Information
ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.