Proteintech, 67735-1-Ig-150ul, Alpha smooth muscle actin specific Monoclonal antibody

67735-1-Ig targets Alpha smooth muscle actin specific in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
Tested Applications
Positive WB detected in: rat stomach tissue, rabbit stomach tissue, pig vein tissue, pig stomach tissue, rabbit large intestine tissue, pig colon tissue, rat colon tissue, mouse colon tissue
Positive IHC detected in: human colon tissue, human appendicitis tissue, human liver tissue, human liver cancer tissue, rat colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: rat heart tissue
Positive IF-Fro detected in: mouse breast cancer
Positive IF/ICC detected in: C2C12 cells
Positive FC (Intra) detected in: C2C12 cells
Recommended dilution
Western Blot (WB): WB : 1:20000-1:100000
Immunohistochemistry (IHC): IHC : 1:2500-1:10000
Immunofluorescence (IF)-P: IF-P : 1:200-1:800
Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Background Information
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.