Proteintech, 67742-1-Ig-20ul, ACADM Monoclonal antibody

67742-1-Ig targets ACADM in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, pig samples.
Tested Applications
Positive WB detected in: PC-3 cells, HSC-T6 cells, rat liver tissue, HeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected in: human liver tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: human liver cancer tissue
Recommended dilution
Western Blot (WB): WB : 1:2000-1:10000
Immunohistochemistry (IHC): IHC : 1:250-1:1000
Immunofluorescence (IF)-P: IF-P : 1:200-1:800
Background Information
ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.