Proteintech, 67768-1-Ig-150ul, ALCAM Monoclonal antibody

67768-1-Ig targets ALCAM in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: HT-1080 cells, U2OS cells, A549 cells, LNCaP cells
Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: human breast cancer tissue
Positive IF-Fro detected in: mouse breast cancer
Positive IF/ICC detected in: hTERT-RPE1 cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunohistochemistry (IHC): IHC : 1:500-1:2000
Immunofluorescence (IF)-P: IF-P : 1:200-1:800
Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
Background Information
Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).