Proteintech, 68113-1-Ig-150ul, ADAM8 Monoclonal antibody

68113-1-Ig targets ADAM8 in WB, FC, ELISA applications and shows reactivity with human, pig samples.
Tested Applications
Positive WB detected in: human testis tissue, HaCaT cells, A431 cells, pig stomach tissue, pig pancreas tissue
Positive FC detected in: A431 cells
Recommended dilution
Western Blot (WB): WB : 1:1000-1:4000
Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Background Information
ADAM8 is a type I transmembrane (TM) glycoprotein consisting of an N-terminal prodomain, followed by a metalloproteinase-, disintegrin (DIS)-, cysteine-rich-, epidermal growth factor (EGF)-like- and TM domain and a cytoplasmic tail. Expression levels of ADAM8 in normal tissue are typically low and limited to a few distinct cell types in the lymphatic organs as components of the immune system, the central nervous system, bone, and the lung. This antibody can be used to detect endogenous ADAM8 with an apparent molecular weight of 90 kDa (the calculated MW ). ADAM8 also can be detectable as a band with a molecular weight (MW) of 98 kDa, slightly larger than the calculated MW (90 kDa) (PMID:11050116).