Proteintech, 68140-1-Ig-150ul, ACOT7 Monoclonal antibody

68140-1-Ig targets ACOT7 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse, Rabbit, Pig samples.
Tested Applications
Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Background Information
Acyl-CoA thioesterases (ACOTs) are enzymes that catalyze hydrolysis of fatty acyl-CoAs to free fatty acids and CoA-SH. ACOTs are expressed ubiquitously in prokaryotes and eukaryotes (PMID: 11755680). ACOT7 also known as BACH, CTE-II, ACT, ACH1, BACHa, is one of the most extensively studied ACOTs as a major isoform of the ACOT family (PMID: 17563367). The enzyme is localized to the cytosol and is highly expressed in brain tissue and testis tissue, showing a preference for long-chain acyl-CoA substrates with fatty acid chains of 8-16 carbon atoms (C8-C16) (PMID: 28518146). It has been identified that ACOT7 plays a role in neurotoxic prevention and is a candidate drug target in inflammatory disease (PMID: 17563367, 23459938, 10509865). ACOT7 has 7 isoforms as molecular between 27-40 kDa, it may also form dimer (~70 kDa) (PMID: 20470824).