Proteintech, 68223-1-Ig-150ul, ACTN2 Monoclonal antibody

68223-1-Ig targets ACTN2 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
Tested Applications
Positive WB detected in: pig heart tissue, rat colon tissue, rabbit heart tissue, rat heart tissue, mouse heart tissue, pig colon tissue
Recommended dilution
Western Blot (WB): WB : 1:20000-1:100000
Background Information
Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).