Proteintech, 68301-1-Ig-20ul, 5-methylcytosine Monoclonal antibody

68301-1-Ig targets 5-methylcytosine in WB, IHC, IF, RIP, Dot Blot, ELISA applications and shows reactivity with 5mc, chemical compound, m5c samples.
Tested Applications
Positive IHC detected in: mouse testis tissue, rat brain tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive ELISA detected in: compound
Positive Dot Blot detected in: HeLa cells
Recommended dilution
Immunohistochemistry (IHC): IHC : 1:2500-1:10000
Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:10-1:100
DOT BLOT: DOT BLOT : 1:2500-1:10000
Background Information
In eukaryotes, 5-methylcytosine in DNA (5mC) and its oxidized derivatives (5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC)) are the most prominent modifications and have been suggested to contribute to epigenetic gene regulation through a variety of different mechanisms. m5C has also been found in mRNA, rRNA and tRNA of representative organisms from all kinds of species. As reversible epigenetic modifications, m5C modifications of RNA affect the fate of the modified RNA molecules and play important roles in various biological processes including RNA stability control, protein synthesis, and transcriptional regulation (PMID: 35365216).
This antibody can recognize both 5mC in DNA and m5C in RNA.Protocol for Dot Blot:https://www.ptglab.com/protocol/68301-1-IgDotBlot.pdf