Description
68332-1-Ig targets ALAS2 in WB, ELISA applications and shows reactivity with Human samples.
Tested Applications
Positive WB detected in: THP-1 cells, HEK-293 cells, Daudi cells, NK-92 cells, Raji cells, MOLT-4 cells, K-562 cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Background Information
The heme biosynthetic pathway begins from delta aminolevulinic acid synthase (ALAS) catalyzing the condensation of glycine and succinyl-CoA to delta aminolevulinic acid (ALA) in the mitochondria. ALAS is coded by two genes: ALAS1 and ALAS2 . ALAS1 is ubiquitously expressed in all cells, and the negative feedback is regulated by the heme pool; however, ALAS2 is specifically expressed only in erythroid cells.