Proteintech, 68579-1-Ig-20ul, 5-Hydroxymethylcytidine Monoclonal antibody

68579-1-Ig targets 5-Hydroxymethylcytidine in Dot Blot, ELISA applications and shows reactivity with chemical compound samples.
Tested Applications
Positive ELISA detected in: 5-Hydroxymethylcytidine
Positive Dot Blot detected in: RNA
Recommended dilution
Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:2000-1:20000
DOT BLOT: DOT BLOT : 1:1000-1:4000
Background Information
Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. Recent reaearch shows that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA. It is known that RNA carries more than 100 distinct types of modifications, and these modifications modulate the structure and functions of RNA. Ribonucleoside 5-methylcytidine (m5C) is subject to oxidative processing in mammals, forming 5-hydroxymethylcytidine (hm5C) and 5-formylcytidine (f5C). Researchers have identified hm5C in total RNA from all three domains of life and in polyA-enriched RNA fractions from mammalian cells. This suggests m5C oxidation is a conserved process that could have critical regulatory functions inside cells (PMID: 25676849).Protocol for Dot Blot:https://www.ptglab.com/protocol/68579-1-IgDotBlot.pdf