Proteintech, 68618-1-Ig-150ul, Angiopoietin 1 Monoclonal antibody

68618-1-Ig targets Angiopoietin 1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications
Positive WB detected in: A375 cells, PNGase F treated A375 cells, non-treated U-87 MG cells, PNGase F treated U-87 MG cells, non-treated HuH-7 cells, PNGase F treated HuH-7 MG cells, non-treated bENd.3 cells, PNGase F treated bENd.3 cells
Positive IF/ICC detected in: HUVEC cells
Recommended dilution
Western Blot (WB): WB : 1:2000-1:10000
Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
Background Information
Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.