Proteintech, 68725-1-Ig-150ul, ADAM17 Monoclonal antibody

68725-1-Ig targets ADAM17 in WB, ELISA applications and shows reactivity with Human, Mouse samples.
Tested Applications
Positive WB detected in: U2OS cells, NIH/3T3 cells, Calu-3 cells, A549 cells, HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:2000-1:10000
Background Information
The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).