Proteintech, 80008-1-RR-20ul, Alpha smooth muscle actin specific Recombinant monoclonal antibody

80008-1-RR targets Alpha smooth muscle actin specific in WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, chicken, zebrafish samples.
Tested Applications
Positive WB detected in: mouse colon tissue, pig vein tissue, chicken crop tissue, zebrafish tissue, A549 cells, pig stomach, pig colon, rat stomach, chicken small intestine tissue, rat colon tissue
Positive IHC detected in: human liver tissue, human colon tissue, mouse colon tissue, rat colon tissue, human heart tissue, human appendicitis tissue, human rectal cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: mouse heart tissue, mouse embryo tissue
Positive IF-Fro detected in: mouse small intestine tissue
Positive FC (Intra) detected in: C2C12 cells
Recommended dilution
Western Blot (WB): WB : 1:20000-1:100000
Immunohistochemistry (IHC): IHC : 1:2500-1:10000
Immunofluorescence (IF)-P: IF-P : 1:500-1:2000
Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
Background Information
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.