Proteintech, 81844-1-RR-20ul, androgen receptor Recombinant monoclonal antibody

81844-1-RR targets androgen receptor in WB, IHC, IF/ICC, ELISA, ChIP-qPCR applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: LNCaP cells, MCF-7 cells, K-562 cells
Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: LNCaP cells
Positive ChIP-qPCR detected in: dihydrotestosterone (DHT, 10 nM) for 4 hours LNCaP cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunohistochemistry (IHC): IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
CHIP-QPCR: CHIP-QPCR : 1:10-1:100
Background Information
Androgen receptor (AR) is a steroid hormone receptor for androgenic hormones such as 17β-Hydroxy-3-oxo-4-androstene and DHT. AR plays a vital role in developing and maintaining male sex phenotypes as well as an additional role in regulating bone metabolism.1. What is the molecular weight of AR? Are there any isoforms of AR?The molecular weight of full-length androgen receptor (AR-B) is 110 kDa. An additional variant, AR-A, has an 87 kDa size and lacks the N-terminal 187 amino acids of AR-A (PMID: 8108393). Recently, more splice variants of AR have been discovered, raising protein products of around 80 kDa length (PMID: 19244107), as well as an AR45 variant of 45 kDa size (PMID: 15634333). AR splice variants differ in their cell line-specific expression (PMID: 24570075).2. What is the subcellular localization of AR?AR can be present in either or both of the cytoplasm and nucleus. In androgen-deprived cells, AR is found predominantly in the cytoplasm, while stimulation by androgens causes enrichment of androgen-bound AR in the nucleus. AR shuttles between the cytoplasm and nucleus and its phosphorylation state has an impact on subcellular localization (PMID: 16282370).3. Is AR post-translationally modified?Post-translational modifications of the AR include phosphorylation, acetylation, methylation, SUMOylation, and ubiquitination (PMID: 21820033). These modifications have an impact on receptor stability, activity, and can change the observed molecular weight of the AR.4. How to study AR signaling in cell culture?It is important to control levels of cell stimulation while also looking at AR signaling. Fetal bovine serum (FBS) that is typically used in cell culture contains low levels of 17β-Hydroxy-3-oxo-4-androstene that are enough to stimulate the growth of prostate cells (PMID: 19676093), including the LNCaP cell line that is a commonly used human prostatic carcinoma cell model (PMID: 6831420). One possibility for complete 17β-Hydroxy-3-oxo-4-androstene deprivation is to use charcoal stripped FBS that removes lipophilic agents, including androgens. It is also not recommended to use phenol red in your medium because it is a weak estrogen (PMID: 3458212). Cell stimulation is often conducted by DHT.5. What is the role of AR in prostate cancer?AR plays a key role in the development and physiology of the prostate gland, and also cancer progression (PMID: 15082523). Mutations in AR altering ligands have been observed. The progression of the prostate cancer depends on AR activity and therefore blocking AR activity or lowering androgen levels is a key step related to androgen deprivation therapy (ADT).