Proteintech, 82083-1-RR-100ul, ALKBH5 Recombinant monoclonal antibody

82083-1-RR targets ALKBH5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, A549 cells, K-562 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected in: U-251 cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
Background Information
ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.