Proteintech, 82122-5-RR-20ul, ACOT2 Recombinant monoclonal antibody

82122-5-RR targets ACOT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: L02 cells, U-87 MG cells, HEK-293 cells, HepG2 cells, RT-4 cells
Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: HepG2 cells
Recommended dilution
Western Blot (WB): WB : 1:2000-1:10000
Immunohistochemistry (IHC): IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:500
Background Information
Acyl-CoA thioesterase (Acot)2 localizes to the mitochondrial matrix and hydrolyses long-chain fatty acyl-CoA into free FA and CoASH. Acot2 is expressed in highly oxi­dative tissues and is poised to modulate mitochondrial FA oxidation (FAO) (PMID: 25114170). The structure of ACOT2 consists of two domains, N and C domains, and the active site of ACOT2 is located at the interface between the N and C domains (PMID: 19497300).