Proteintech, 82714-5-RR-20ul, ACTA1-Specific Recombinant monoclonal antibody

82714-5-RR targets ACTA1-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, chicken samples.
Tested Applications
Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue, pig skeletal muscle tissue, chicken skeletal muscle tissue
Positive IHC detected in: mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: mouse skeletal muscle tissue
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunohistochemistry (IHC): IHC : 1:500-1:2000
Immunofluorescence (IF)-P: IF-P : 1:50-1:500
Background Information
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.