Proteintech, 82828-14-RR-20ul, Acetyl-Histone H3 (Lys36) Recombinant monoclonal antibody

82828-14-RR targets Acetyl-Histone H3 (Lys36) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.
Tested Applications
Positive WB detected in: Trichostatin A treated NIH/3T3 cells
Positive IF/ICC detected in: Trichostatin A treated NIH/3T3 cells
Positive Dot Blot detected in: N/A N/A
Positive ChIP-qPCR detected in: HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:500-1:2000
Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
DOT BLOT: DOT BLOT : 1:10-1:100
CHIP-QPCR: CHIP-QPCR : 1:10-1:100
Background Information
H3K36ac peaks at the 5' end of genes,manily on the two nucleosomeus immediately distal to the transcription start site, independently of gene length. H3K36ac and H3K36mac showed negative creastalk, which is mediated by GCN3 and the histone methyl transferase SDX8. Although H3K36ac is associated with gene activity, there is no linear relationship between H3K36ac and transcript levels, suggesting that H3K36ac is a binary indicator of transcription.