Proteintech, 82832-1-RR-100ul, Acetyl-Histone H3 (Lys18) Recombinant monoclonal antibody

82832-1-RR targets Acetyl-Histone H3 (Lys18) in WB, ChIP, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: Trichostatin A treated NIH/3T3 cells, HSC-T6 cells, NIH/3T3 cells
Positive Dot Blot detected in: peptide
Positive ChIP-qPCR detected in: HeLa cells
Positive ChIP detected in: HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
DOT BLOT: DOT BLOT : 1:10-1:100
CHIP-QPCR: CHIP-QPCR : 1:10-1:100
Chromatin immunoprecipitation (ChIP): CHIP : 1:10-1:100
Background Information
Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.