Proteintech, 82974-1-RR-20ul, AIFM2/ FSP1 Recombinant monoclonal antibody

82974-1-RR targets AIFM2/ FSP1 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: HeLa cells, HepG2 cells
Positive IP detected in: HepG2 cells
Positive FC (Intra) detected in: A549 cells
Recommended dilution
Western Blot (WB): WB : 1:2000-1:16000
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Background Information
The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.