Proteintech, 83041-1-RR-100ul, Acetyl-Histone H2A (Lys9) Recombinant monoclonal antibody

83041-1-RR targets Acetyl-Histone H2A (Lys9) in WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.
Tested Applications
Positive WB detected in: Trichostatin A treated NIH/3T3 cells
Positive IHC detected in: mouse lung tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: HeLa cells
Positive Dot Blot detected in: peptide
Positive ChIP-qPCR detected in: HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:2000-1:10000
Immunohistochemistry (IHC): IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
DOT BLOT: DOT BLOT : 1:10-1:100
CHIP-QPCR: CHIP-QPCR : 1:10-1:100
Background Information
Histone H2A is a core component of nucleosome. Histone variants contribute to chromatin complexity by creating specialized nucleosomes. Within nucleosomes, either one canonical H2A or both of them can be exchanged with a particular variant (heterotypic and homotypic nucleosomes, respectively), and such changes can have profound influences on nucleosome stability and biological outcome.