Proteintech, 83095-1-RR-20ul, Acetyl-Histone H4 (Lys12) Recombinant monoclonal antibody

83095-1-RR targets Acetyl-Histone H4 (Lys12) in WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: Trichostatin A treated NIH/3T3 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected in: mouse small intestine tissue, human placenta tissue, mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: Caco-2 cells, Trichostatin A treated NIH/3T3 cells
Positive Dot Blot detected in: peptide
Positive ChIP-qPCR detected in: HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunohistochemistry (IHC): IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600
DOT BLOT: DOT BLOT : 1:10-1:100
CHIP-QPCR: CHIP-QPCR : 1:10-1:100
Background Information
Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication.