Proteintech, 83890-5-RR-20ul, 4EBP1 Recombinant monoclonal antibody

83890-5-RR targets 4EBP1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: A549 cells, K-562 cells, HL-60 cells, HepG2 cells, HeLa cells, A431 cells
Positive FC (Intra) detected in: HEK-293 cells, HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Background Information
4EBP1 (4E-BP1) is a translational regulator and functions by sequestering eukaryotic translation initiation factor 4E (eIF4E) from the translation initiation machinery. 4E-BP1 contains at least six phosphorylation sites (Thr37, Thr46, Ser65, Thr70, Ser83, and Ser112), four of which (Thr37, Thr46, Ser65, and Thr70) are known to be regulated by mTOR signaling. Phosphorylation of Thr37 and Thr46 is thought to prime 4E-BP1 for sequential phosphorylation of Ser65 and Thr70, which results in the dissociation of 4E-BP1 from eIF4E. (PMID: 12747827)