Proteintech, 83912-5-RR-20ul, ANK2 Recombinant monoclonal antibody

83912-5-RR targets ANK2 in IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Applications
Positive IHC detected in: mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: mouse heart tissue
Positive FC (Intra) detected in: HEK-293 cells
Recommended dilution
Immunohistochemistry (IHC): IHC : 1:200-1:800
Immunofluorescence (IF)-P: IF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Background Information
The Ankyrins, belonging to the adapter protein family, consist of Ankyrin-R (ANK1), Ankyrin-B (ANK2), and Ankyrin-G (ANK3). Initially, ANK2 was considered as a key cytoskeletal-associated protein in the brain. Besides, it was discovered as a pivotal adaptor and scaffolding protein in the heart. Remarkably, recent studies have demonstrated that Ankyrins contribute to the modulation of various biological activities in cancers. (PMID: 36539782)