Description
84314-2-RR targets ALKBH3 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: A375 cells, mouse kidney tissue, SGC-7901 cells, mouse heart tissue, rat heart tissue
Positive FC (Intra) detected in: PC-3 cells, HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Background Information
ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This antibody specifically recognizes isoform 1 as 33kDa (Uniprot). It is possible that there is ubiquitination modification (PMID: 25944111).