Proteintech, 84551-1-RR-100ul, Acetyl-Histone H2B (Lys120) Recombinant monoclonal antibody

84551-1-RR targets Acetyl-Histone H2B (Lys120) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.
Tested Applications
Positive WB detected in: Trichostatin A treated NIH/3T3 cells
Positive IF/ICC detected in: Trichostatin A treated HeLa cells
Positive Dot Blot detected in: peptide
Positive ChIP-qPCR detected in: HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:500-1:2000
Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
DOT BLOT: DOT BLOT : 1:10-1:100
CHIP-QPCR: CHIP-QPCR : 1:10-1:100
Background Information
Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).