Proteintech, 84686-4-RR-20ul, AEG-1/MTDH Recombinant monoclonal antibody

84686-4-RR targets AEG-1/MTDH in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: A549 cells, HeLa cells, HL-60 cells, MCF-7 cells, HepG2 cells, U-937 cells
Positive IF/ICC detected in: A431 cells
Positive FC (Intra) detected in: A549 cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Background Information
MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression.