Proteintech, 85379-1-RR-100ul, ACADL-Specific Recombinant monoclonal antibody

85379-1-RR targets ACADL-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: mouse liver tissue, mouse kidney tissue, mouse lung tissue, rat liver tissue
Positive IHC detected in: mouse brain tissue, mouse liver tissue, rat liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunohistochemistry (IHC): IHC : 1:250-1:1000
Background Information
ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065). Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).This antibody is specific to ACADL.