Proteintech, 86134-1-RR-20ul, Angiotensinogen/AGT Recombinant monoclonal antibody

86134-1-RR targets Angiotensinogen/AGT in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.
Tested Applications
Positive WB detected in: mouse heart tissue, rat liver tissue, mouse brain tissue, mouse lung tissue, mouse kidney tissue
Positive IHC detected in: mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:2000-1:10000
Immunohistochemistry (IHC): IHC : 1:250-1:1000
Background Information
Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.