Proteintech, 86340-2-RR-20ul, Alpha Actinin Recombinant monoclonal antibody

86340-2-RR targets Alpha Actinin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: HeLa cells, PANC-1 cells, NIH/3T3 cells, C6 cells, mouse spleen tissue, rat heart tissue
Positive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: mouse heart tissue
Positive IF/ICC detected in: H9C2 cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunohistochemistry (IHC): IHC : 1:750-1:3000
Immunofluorescence (IF)-P: IF-P : 1:250-1:1000
Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
Background Information
Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.