Proteintech, 86731-1-RR-100ul, Acetyl-Histone H2B (Lys12) Recombinant monoclonal antibody

86731-1-RR targets Acetyl-Histone H2B (Lys12) in WB, IHC, Dot blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.
Tested Applications
Positive WB detected in: Trichostatin A treated NIH/3T3 cells, Trichostatin A treated HeLa cells
Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive Dot blot detected in: / /
Positive ChIP-qPCR detected in: HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunohistochemistry (IHC): IHC : 1:500-1:2000
DOT BLOT: DOT BLOT : 1:10-1:100
CHIP-QPCR: CHIP-QPCR : 1:10-1:100