Proteintech, 87067-1-RR-100ul, ADO Recombinant monoclonal antibody

87067-1-RR targets ADO in WB, ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: HEK-293 cells, HeLa cells, A-673 cells, PC-3 cells, fetal human brain tissue
Recommended dilution
Western Blot (WB): WB : 1:2000-1:10000
Background Information
ADO, or 2-aminoethanethiol dioxygenase, cysteamine dioxygenase, is a thiol dioxygenase in humans that adds two oxygen atoms to cysteamine, forming hypotaurine (PMID: 39637922). ADO has been reported to exhibit two distinct biological functions with a nonheme iron center. It catalyzes oxidation of both cysteamine in sulfur metabolism and N-terminal cysteine-containing proteins or peptides, such as regulator of G protein signaling 5 (RGS5) (PMID: 34508780). ADO is ubiquitously expressed with the highest levels in brain, heart, and skeletal muscle. Western blot analysis detected two bands at 28~30 kDa as indicated in publications (PMID: 17581819).