Description
The QIAprep 96 Turbo Kits provide QIAprep 96 and TurboFilter 96 plates for purification of up to 20 µg molecular biology grade plasmid DNA per well. For optimal results it is recommended to use this product together with QIAvac 96.
The QIAprep 96 Turbo Kits are designed for isolation of up to 20 µg pure plasmid or cosmid DNA for use in routine molecular biology applications, such as sequencing and cloning (see figure "High-quality DNA for automated sequencing"). In addition to plasmid purification from Escherichia coli, QIAprep 96 Turbo Kits can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact QIAGEN Technical Services or your local distributor for protocols for these applications.
QIAprep 96 plates contain a unique silica membrane that binds up to 20 µg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time consuming phenol–chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. Pure plasmid DNA eluted from QIAprep 96 plates is immediately ready to use — there is no need to precipitate, concentrate, or desalt. TurboFilter 96 plates are specially designed to allow fast clearing of bacterial lysates generated under high concentrations of chaotropic salts. Crude bacterial lysates are vacuum filtered through the TurboFilter 96 plate, eliminating the need for centrifugation. Particle-free lysates flow directly into the wells of the QIAprep 96 plate, ready for purification and elution. TurboFilter technology streamlines QIAprep plasmid purification and allows the procedure to be fully automated on the BioRobot Universal System. Specifications Features QIAprep 96 Turbo Miniprep Kit QIAprep 96 Turbo BioRobot Kit Applications Fluorescent and radioactive sequencing, cloning, etc. Fluorescent and radioactive sequencing, cloning, etc. Culture volume/starting material 1–10 ml culture volume 1–10 ml culture volume Plasmid type High-copy, low-copy, cosmid DNA High-copy, low-copy, cosmid DNA Processing Manual (vacuum) Manual (vacuum) Samples per run; throughtput 48–96 samples per run 48–96 samples per run Technology Silica technology Silica technology Time per run <45 min/plate <70 minutes Yield <20 µg <20 µg
Plasmid purification using QIAprep 96 Turbo Kits follow a simple bind–wash–elute procedure (see flowchart "The QIAprep procedure"). First, bacterial cultures are lysed and the lysates are cleared by filtration through TurboFilter modules. The cleared lysates are then applied to the QIAprep 96 plate where plasmid DNA adsorbs to the silica membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water. The QIAprep 96 Turbo procedure is performed entirely in 96-well format, reducing sample handling to a minimum, allowing 96 parallel minipreps to be processed in 45–70 minutes. Bacterial cultures can be grown and lysed in the 96-well blocks provided with the kit. The QIAvac 96 manifold is designed for in-line filtration in 96-well format, so that cleared lysates from the TurboFilter 96 plate flow directly into the wells of the QIAprep 96 plate, providing high-throughput of 96 samples in parallel.The QIAprep 96 Turbo BioRobot Kit is a special kit format optimized on the BioRobot 8000 (no longer available) or the BioRobot Universal System. The kit provides all components required for automated preparation of 96 high-purity plasmid minipreps.
