Description
Puregene Accessories used in combination with Puregene Kits enable purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows large-volume samples to be purified for use as long-term references. To improve availability address demand more efficiently we implemented an in-house manufacturing solution. This leads to product name and catalogue number changes. This Change will not reflect on the quality and performance of the products. The legacy products will be discontinued on 28.02.2022 and be available only until stocks last. Please use the table below in the Performance section to find the product you need.
Catalog number changes in the Puregene line. NEW product name NEW cat.no. Old product name Old cat. no. 158103 Puregene RBC Lysis Solution (450 ml) 158902 RBC Lysis Solution(450 ml) 158106 Puregene RBC Lysis Solution (1000 ml) 158904 RBC Lysis Solution (1000 ml) 158113 Puregene Cell Lysis Solution (125 ml) 158906 Cell Lysis Solution (125 ml) 158116 Puregene Cell Lysis Solution (1000 ml) 158908 Cell Lysis Solution (1000 ml) 158123 Puregene Protein Precipitation Sol. (50 ml) 158910 Protein Precipitation Solution (50 ml) 158126 Puregene Protein Precipitation Solution (350 ml) 158912 Protein Precipitation Solution (350 ml) 158133 Puregene DNA Hydration Solution (100 ml) 158914 DNA Hydration Solution (100 ml) 158136 Puregene DNA Hydration Solution (500 ml) 158916 DNA Hydration Solution (500 ml) 158143 Proteinase K (650 µl) 158918 Puregene Proteinase K (650 µl) 158146 Proteinase K (5 ml) 158920 Puregene Proteinase K (5 ml) 158153 RNase A Solution (650 µl) 158922 RNase A Solution (650 µl) 158156 RNase A Solution (5 ml) 158924 RNase A Solution (5 ml) 158183 Glycogen Solution (500 µl) 158930 Glycogen Solution (500 µl) 158186 Glycogen Solution (5 ml) 949002 Autopure Glycogen Solution (5 ml)
Using Puregene Kits, cells are lysed with an anionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in hydration solution (1 mM EDTA, 10 mM Tris·Cl pH 7.5). Purified DNA typically has an A 260 / A 280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2–8°C, –20°C, or –80°C.
Archive-quality DNA Purity of DNA has a significant effect on the accuracy of results obtained in downstream applications. Sensitive downstream applications, such as PCR, demand the use of DNA of the highest quality and molecular weight for reliable results. Puregene Kits removes contaminants and enzyme inhibitors enabling purification of highly stable DNA well-suited for archiving. Purified DNA is of high quality demonstrated by molecular weights of 100–200 kb.
