Description
With the QIAsymphony DSP DNA Mini Kit, in combination with the QIAsymphony GeneRead DNA FFPE Treatment Kit and QIAsymphony SP, it is possible to fully automate DNA extraction from up to 96 FFPE samples in parallel. Enzymatic removal of cytosine deamination artifacts from FFPE samples in combination with optimized protocols provide high yields of pure DNA, suitable for downstream sequencing applications in the GeneReader NGS System workflow. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.
The QIAsymphony DSP DNA mini kit in combination with the QIAsymphony GeneRead DNA FFPE Treatment Kit can be used to purify DNA from FFPE tissues. This combines the speed and efficiency of silica-based purification of DNA with the convenient handling of magnetic particles (see figure "Schematic of the QIAsymphony SP principle"). Innovative, ready-to-run reagent cartridges are prefilled with all reagents required for the purification procedure and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure "Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves you valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.
The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute (see figure "QIAsymphony DSP DNA procedure"). Optimized protocols provide high quality nucleic acids with efficient removal of proteins, nucleases, and other impurities. Nucleic acids purified with QIAsymphony GeneRead DNA FFPE Treatment Kit and QIAsymphony DSP DNA Mini Kit is ready for direct use in downstream library preparation and sequencing applications on the GeneReader NGS System. The user can choose between different elution volumes.
