Description
The MagAttract 96 DNA Plant Core Kit combines the efficiency of silica-based DNA purification with the convenient handling offered by magnetic particles, in manual, semi-automated, or automated 96-well plate format.
Purification using the MagAttract 96 DNA Plant Core Kit Format: 96-well sample plates Sample sources: Plant tissue Plant starting material, fresh or frozen: 10–100 mg Plant starting material, dried or lyophilized: 10–30 mg Typical yield: 1–15 µg Elution volume: 100 µl Purified DNA has been used successfully in PCR and real-time PCR (see figures "Successful PCR analysis "and "Reliable real-time PCR analysis").
The MagAttract 96 DNA Plant Kit is designed for fully automated, high-throughput preparation of genomic and chloroplast DNA from plant tissue. Proven MagAttract magnetic particle technology in combination with the BioRobot Plant Science system (no longer available) provides efficient and reproducible DNA purification (see figure "Reproducible yields").
Plant samples are mechanically disrupted using a homogenizer such as the TissueLyser. The resulting powder is resuspended in lysis buffer, thoroughly mixed, and centrifuged. Lysates are transferred to a 96-well microplate containing MagAttract magnetic particles, and genomic DNA selectively binds to the surface of the particles (see "MagAttract 96 DNA Plant Core procedure"). After washing, pure genomic DNA is eluted with low-salt buffer, and is ready for use in downstream applications. The MagAttract 96 DNA Plant Kit is designed for use with the BioRobot Plant Science system for fully automated DNA purification. QIAGEN also offers an automated protocol using the BioRobot RapidPlate workstation, and a manual protocol for evaluation purposes.
