Qiagen, 20021681, Verogen MiSeq FGx Reagent Micro Kit

Name: Verogen MiSeq FGx Reagent Micro Kit
Brand: Qiagen
SKU: 20021681

Supports up to 5 million paired-end reads for small batch sizes and faster turnaround times

Catalog Number: 20021681

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Category

NGS Library Preparation Kits

TagsHuman ID & Forensics Next-Generation Sequencing for HID

Description

Generate high-quality sequencing data with MiSeq FGx Sequencing Reagents. These pre-filled, ready-to-use cartridges leverage gold-standard sequencing-by-synthesis (SBS) chemistry to support a range of laboratory throughput requirements. MiSeq FGx Sequencing Reagents support both Forensic Genomics and Research Use Only modes on the MiSeq FGx Sequencing System to simplify inventory and ordering for all applications.
The MiSeq FGx Reagent Kit has been optimized for sequencing forensic samples and delivers high-quality performance for a range of forensic applications. The extended read lengths enable deep sequencing of STRs, mtDNA and SNPs. The MiSeq FGx Reagent Micro Kit is purpose-built for lower throughput and faster sequencing time, while delivering equivalent quality. Both kit sizes are quality controlled to meet the high standards expected from forensic laboratories. The MiSeq FGx Sequencing Reagents are integral to the MiSeq FGx Sequencing System and support analysis of libraries across the range of Verogen NGS kits.
The MiSeq FGx System achieves exceptional data quality by employing a proprietary, reversible terminator‑based method that detects single bases as they are incorporated into massively parallel DNA strands. Fluorescent terminator dyes are imaged as each deoxynucleotide triphosphate (dNTP) is added and then cleaved to allow incorporation of the next base. With all four reversible, terminator-bound dNTPs present at each sequencing cycle, natural competition among bases minimizes incorporation bias. The MiSeq FGx Reagent Micro Kit and the MiSeq FGx Reagent Kit are identical in manufacture. With both kits, the best-in-class sequencing-by-synthesis (SBS) technology remains unaltered. The only difference is the surface area of the flow cell available for imaging. The MiSeq FGx System images the micro flow cell at approximately 35% of the standard flow cell area for a total output of 5 million reads.
The first sequencing step of the MiSeq FGx Sequencing System uses a library prep kit to add primers to genomic DNA (gDNA), mtDNA, or mRNA extracted from forensic samples for simultaneous capture and amplification of hundreds of target regions, generating dual-indexed libraries ready for sequencing. After library prep, load-and-go MiSeq FGx Sequencing Reagents streamline run setup. Simply thaw the prefilled reagent cartridge, add libraries to the cartridge, and insert the cartridge into the instrument. The system software accepts run parameters and starts sequencing with the push of a button.

FAQs

Question: How many cycles are included?

Answer:The Micro Kit provides 600 sequencing cycles.

Question: What is the maximum read length?

Answer:The kit supports paired-end sequencing up to 2 × 300 bp.

Question: What read capacity does the Micro Kit provide?

Answer:It supports up to approximately 5 million paired-end reads.

Application Note

The Verogen MiSeq FGx Reagent Micro Kit is a lower-throughput sequencing consumable set for the MiSeq FGx System. The 600-cycle format supports paired-end sequencing up to 2 × 300 bp and can produce up to approximately 5 million paired-end reads with a maximum output of about 5 Gb. It supplies the cluster-generation, sequencing-by-synthesis, and paired-end chemistry required for a complete run and uses a prefilled cartridge to streamline instrument setup. The Micro format is useful for smaller forensic sample batches, pilot experiments, and workflows that prioritize faster turnaround over the higher read capacity of the standard MiSeq FGx reagent kit. It supports compatible DNA, RNA, and mitochondrial DNA libraries. Selecting the Micro Kit can reduce unused sequencing capacity when sample numbers or target depth are modest.

Ordering

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