Qiagen, 205211, Sensiscript RT Kit (50)

Name: Sensiscript RT Kit (50)
Brand: Qiagen
SKU: 205211

For 50 reverse-transcription reactions: Sensiscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1.1 ml RNase-free water

Catalog Number: 205211

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Category

Reverse Transcriptases

TagsDiscovery & Translational Research PCR/qPCR/dPCR PCR Enzymes & Kits Reverse Transcription & cDNA Synthesis

Description

The Sensiscript Reverse Transcriptase (RT) Kit is ideally suited for highly sensitive applications using very small amounts of RNA (less than 50 ng), such as single-cell RT-PCR and analysis of biopsies and LMD samples. Sensiscript Reverse Transcriptase allows highly efficient RT-PCR over a wide dynamic range and extremely sensitive RT-PCR with very small RNA amounts due to its high affinity for RNA. The Sensiscript RT Kit includes Sensiscript Reverse Transcriptase, dNTPs, and an optimized reaction buffer. Simply add primers for fast and easy cDNA synthesis.
Sensiscript Reverse Transcriptase (RT) provides outstanding efficiency over a wide dynamic range and high sensitivity for very small amounts of RNA (see figure "RT-PCR with RNA corresponding to 1 cell"). Regions of RNA with high GC content can cause other reverse transcriptases to stop, dissociate from the RNA template, or skip over looped-out regions of RNA (see figure Full-length RT-PCR products — B). These difficult templates, however, prove no problem for QIAGEN reverse transcriptases (see figure Full-length RT-PCR products — A). With no need for optimization, the Sensiscript RT Kit makes reverse transcription virtually trouble-free. Lot-to-lot reproducibility of Sensiscript RT Kits is ensured by rigorous quality control at QIAGEN. The optimized Buffer RT, dNTPs, and water included in all Sensiscript RT Kits are guaranteed RNase-free, and each lot of Sensiscript RT is thoroughly tested for RT-PCR reproducibility.
Sensiscript Reverse Transcriptase (RT) is the first commercially available enzyme designed for highly sensitive applications with very small amounts of RNA (<50 ng), such as single-cell RT-PCR or analysis of small biopsies. It is provided ready-to-use with dNTPs and in an optimized reaction buffer that, together with the high affinity of Sensiscript RT for RNA, results in highly specific and sensitive RT-PCR, even with low-copy transcripts, and the ability to read through even complex RNA secondary structures without adjusting temperature or reaction conditions. Please note that the primer mix is not provided. For viral RNA, Omniscript RT is usually the enzyme of choice due to the presence of carrier RNA in most viral RNA preparations.
With the optimized Sensiscript Reverse Transcriptase reaction buffer, tedious pipetting and pre-incubation steps are eliminated, and no additional RNase H digestion step is needed (see flowchart "Sensiscript Reverse Transcriptase Procedure").

Specifications

General

Applications	cDNA synthesis, RT-PCR, qRT-PCR
Reaction type	Reverse transcription
Enzyme activity	Reverse transcription
Real-time or endpoint	Endpoint
Mastermix	No
Sample/target type	RNA template
Single or multiplex	Single
With/without hotstart	Without hotstart

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