Description
The Custom Microbial DNA qPCR Arrays are ready-to-use 96- and 384-well plates containing user-selected microbial DNA qPCR Assays for detecting microbial species or microbial genes for your particular need. Master Mix is provided with the arrays. For designing and ordering a Custom Microbial DNA qPCR Array, please go to the custom panel builder in GeneGlobe. Need a quote for your research project or would you like to discuss your project with our specialist team? Just contact us!
Linearity and dynamic range Microbial DNA qPCR Assays display linear amplification across a dynamic range from 10 to 10 6 copies of DNA template (see figure, Linearity and sensitivity of Microbial DNA qPCR Assays). Lower limit of quantification (LLOQ) The LLOQ is the lowest concentration of template that still falls into the linear range of the standard curve (see figure, Limit of detection versus lower limit of quantification). Across all Microbial DNA qPCR Assays, 93% have an LLOQ of <100 gene copies (see figure, The LLOQ for all Microbial DNA qPCR Assays reveals high sensitivity). 92% of microbial identification assays meet this LLOQ, as do 95% of virulence gene assays and 97% of antibiotic resistance gene assays (see figures, The LLOQ for microbial identification Microbial DNA qPCR Assays reveals high sensitivity, The LLOQ for virulence factor gene detection Microbial DNA qPCR Assays reveals high sensitivity, and The LLOQ for antibiotic resistance gene detection Microbial DNA qPCR Assays reveals high sensitivity). Specificity Each Microbial DNA qPCR Assay is stringently tested to ensure that it detects only one target species or gene (see figure, Microbial DNA qPCR Assays are highly specific). For assays that do detect more than one target, a list of detected targets and in silico predictions is included on the product sheet. This specificity is maintained even when samples have high species complexity, such as in stool, sputum, and plaque (see figure, Microbial DNA qPCR Assays display high sensitivity even in complex metagenomic samples), and is verifiable by sequencing methods (see figure, Specificity of the Antibiotic Resistance Genes Microbial DNA qPCR Array is confirmed by pyrosequencing). Reproducibility Microbial DNA qPCR Assays are highly reproducible, both in intra- and inter-individual variability tests (see figure, Microbial DNA qPCR Arrays generate reliably reproducible results).
Microbial DNA qPCR Assays are designed to detect bacterial 16S rRNA gene and fungal ribosomal rRNA gene sequences for species identification, as well as virulence factor genes and antibiotic resistance genes using PCR amplification primers and hydrolysis probe-based detection. Design your own 96- or 384-well microbial DNA assay plate from our selection of predesigned Microbial DNA qPCR Assays. Choose a fully flexible plate layout or select from several convenient predefined layouts. Use our easy-to-use online plate configurator to position the predesigned Microbial DNA qPCR Assays of interest into your plate. The arrays will be delivered ready-to-use, with one single assay per well, sufficient for a 20 µl reaction volume. The Master Mix is provided with the arrays. The table below displays the many different plate layout combinations available for each plate format. Select the plate layout that suits your needs; for example, configure a 96-well plate with 48 assays to be used with 2 samples, or a 96-well plate with 12 assays to be tested with 8 samples. We highly recommend that you include controls on each Custom Microbial DNA qPCR Array. Controls are available for testing for the presence of fungal DNA, bacterial DNA and host genomic DNA and for assessing the success of the PCR reaction, so you can be confident in the results. Plate format Assays x samples per plate Plate pack size (minimum) 96-well plate or Rotor-Disc 100 8 assays x 12 samples 12 plates 12 assays x 8 samples 16 assays x 6 samples 32 assays x 3 samples 24 plates 48 assays x 2 samples 96 assays x 1 samples 384-well plate 8 assays x 48 samples 6 plates 12 assays x 32 samples 16 assays x 24 samples 24 assays x 16 samples 6 plates 32 assays x 12 samples 48 assays x 8 samples 64 assays x 6 samples 96 assays x 4 samples 128 assays x 3 samples 192 assays x 2 samples 24 plates 384 assays x 1 samples
The Microbial DNA qPCR Array procedure is simple and can be carried out in any laboratory with a real-time PCR instrument. DNA is isolated using the QIAamp kit that is suited for the sample type, and then mixed with the appropriate Microbial qPCR Mastermix. The mixture is aliquotted across the array plate, and real-time PCR is performed to obtain the raw C T values for each assay. The complimentary data analysis software is then used to profile genes or species in the sample.
