Description
The Investigator Argus X-12 QS Kit is a multiplex solution for human identification. The 12 X-chromosomal markers, D21S11 and Amelogenin are co-amplified. The autosomal marker, D21S11, enables alignment of the Investigator Argus X-12 QS Kit profile with a profile from any other autosomal STR kit from the expanded CODIS or ESS market, to minimize the risk of sample mix-ups. With its innovative Quality Sensor (QS), the Investigator Argus X-12 QS Kit includes a novel internal performance control. This allows you to readily determine if your PCR run was successful or if there was no DNA present in your sample. The kit utilizes QIAGEN's fast-cycling technology, enabling amplification in just ca. 80 minutes. Highly sensitive, with rapid and reliable results from trace or reference DNA, the Investigator Argus X-12 QS Kit is suitable for paternity or kinship testing and for gonosomal STR analysis of forensic stains. The Investigator Argus X-12 QS Kit meets ISO 18385 requirements.
The Investigator Argus X-12 QS Kit utilizes fast-cycling technology to provide reliable results in approximately 80 minutes. The novel Fast Reaction Mix 2.0 is based on proven QIAGEN Multiplex PCR technology that has been further optimized for direct amplification. Assay performance tests in the presence of increasing concentrations of inhibitors showed superior inhibitor resistance. This means reliable, high-quality results. The Investigator Argus X-12 QS Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode) and the Applied Biosystems 3500 Genetic Analyzer.
The Investigator Argus X-12 QS Kit uses QIAGEN's fast-cycling PCR technology for the direct, simultaneous amplification of 12 X-chromosomal markers, D21S11 and Amelogenin. The amplification of the 12 X-chromosomal markers (see Table 1 and 2), with heightened discrimination power, meets the needs for complex kinship and paternity testing and helps fulfill the demands of complicated deficiency cases involving at least one female. The 12 X-chromosomal markers are clustered into four linkage groups (three markers per group; Table 1), and thus each set of three markers is handled as a haplotype for genotyping. Table 1. Linkage groups Linkage group 1 (Xp22) DXS8378 – DS10135 – DXS10148 2 (Xp11) DXS7132 – DXS10074 – DXS10079 3 (Xp26) HPRTB – DXS10101 – DXS10103 4 (Xp28) DXS7423 – DXS10134 – DXS10146 The innovative Quality Sensors provides additional information on the sample quality and PCR success beyond the profile of your sample. With the Quality Sensor (QS) being amplified as an amplification control, it is possible to determine: A successful PCR run A failed PCR run Lack of DNA in your sample This additional information will help you analyze your data, and guides you with respect to further sample processing. It will also help you reduce unnecessary PCR re-runs, saving you time and money. The additional autosomal marker D12S11 acts as alignment marker. Now, you have the possibility to align your X-chromosomal profile from the Investigator Argus X-12 QS Kit with a profile of any other autosomal STR kit from the expanded CODIS or ESS market, to minimize the risk of sample mix-ups.
The 12 X-chromosomal markers, D21S11 and the gender-specific Amelogenin, are amplified simultaneously in a single PCR run and labeled with one of the dyes listed in the Table 2:"Investigator Argus X-12 QS Kit dyes and corresponding markers". The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as Applied Biosystems GeneMapper ID-X. See the table "Technical specifications "for more information. Table 2. Investigator Argus X-12 QS Kit dyes and corresponding markers Dyes Markers 6-FAM QS1 Amelogenin DXS10103 DXS8378 DXS10101 DXS10134 BTG DXS10074 DXS7132 DXS10135 BTY DXS7423 DXS10146 DXS10079 BTR DXSHPRTB DXS10148 D21S11 Table 3. Investigator Argus X-12 QS Kit technical specifications Category Details Volume per PCR 25 µl Matrix BT5 Fluorescence labels 6-FAM, BTG, BTY, BTR, BTO Genetic analyzers ABI PRISM 3100/3100-Avant, Applied Biosystems 3130/3130 xl or Applied Biosystems 3500/3500xL Genetic Analyzers Software for analysis Applied Biosystems GeneMapper ID, GeneMapper ID-X or Genotyper Software
