Description
ipsogen BCR-ABL1 Mbcr IS-MMR Kits are intended for research use only and are not for use in diagnostic procedures. The kits are for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kits provide reagents optimized for reliable and sensitive detection and quantitation of BCR-ABL p210 b2a2 or b3a2 transcripts, relative to ABL control gene expression.
To ensure highest sensitivity, ipsogen BCR-ABL1 Mbcr IS-MMR Kits have been optimized to detect low levels of BCR-ABL p210 b2a2 or b3a2 transcripts (see figures Accurate detection of Mbcr plasmid standards, high positive RNA control, and IS-MMR calibrator and Reliable detection of ABL plasmid standards, high positive RNA control, and IS-MMR calibrator). The sensitivity has a limit of detection (LOD) of 0.0069.
ipsogen BCR-ABL1 Mbcr IS-MMR Kits are ready-to-use kits for the detection of BCR-ABL p210 b2a2 or b3a2 transcripts using real-time PCR. The kits are based on the amplification and detection of specific BCR-ABL p210 b2a2 or b3a2 transcripts, relative to ABL control gene expression, in total RNA. The kits provide high levels of specificity, sensitivity, and reproducibility. Each ipsogen BCR-ABL1 Mbcr IS-MMR Kit provides 4 standard dilutions for ABL and 5 standard dilutions for Mbcr. Use of a single plasmid for BCR-ABL1 and ABL enables reproducible quantification of transcripts and limits variability. Primers and probe sets are designed according to Europe Against Cancer (EAC) recommendations (see figures Single plasmid for BCR-ABL gene transcript and ABL control gene transcript). The kits also include an IS-MMR calibrator allowing conversion of results to the international scale for accurate measurement of molecular response and major molecular response (MMR) status reporting. A high positive control RNA is also included to monitor the reverse transcription and amplification steps of ABL and BCR-ABL Mbcr during transcript quantification. The ipsogen BCR-ABL1 Mbcr IS-MMR DX Kit includes reagents for reverse transcription-PCR and real-time PCR.
The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of cDNA by real-time PCR. Using ipsogen BCR-ABL1 Mbcr IS-MMR Kits allows detection and quantification of BCR-ABL p210 b2a2 or b3a2 and ABL transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.
