Description
To ensure the highest sensitivity, BCR-ABL1 Mbcr RGQ RT-PCR Kit has been optimized to detect very low levels of BCR-ABL1 p210 b2a2 or b3a2 transcripts. The limit of detection (LOD) is 3.13 CN Mbcr (corresponding to a LOD of 0.0031% with ABL1 CN = 100 000).
BCR-ABL1 Mbcr RGQ RT-PCR Kit is a ready-to-use kit for the detection of BCR-ABL1 p210 b2a2 or b3a2 transcripts using real-time PCR. The kit is based on the amplification and detection of specific BCR-ABL1 p210 b2a2 or b3a2 transcripts, relative to ABL1 control gene expression, in total RNA. The kit provides high levels of specificity, sensitivity, and reproducibility. Each kit provides 4 standard dilutions for ABL1, and 5 standard dilutions for BCR-ABL1 Mbcr. Use of a single plasmid for BCR-ABL1 and ABL1, enables reproducible quantification of transcripts and limits variability. Primers and probe sets are designed according to Europe Against Cancer (EAC) recommendations. The kit also includes an IS-MMR calibrator allowing conversion of results to the International Scale for accurate measurement of molecular response, major molecular response (MMR) and the MR4.5, also called deep molecular response (DMR). The IS-MMR calibrator is included within every run to prevent intra- and inter-lab variation. High and low positive controls RNA are also included to monitor the reverse transcription and amplification steps of ABL1 and BCR-ABL1 Mbcr during transcript quantification.
The BCR-ABL1 Mbcr RGQ RT-PCR Kit provides a comprehensive workflow with results in less than 7 hours from the starting material: RNA extraction from whole blood, reverse transcription of total RNA into cDNA, followed by the amplification and detection of cDNA by real-time PCR on Rotor-Gene Q 5plex HRM, with sensitive enzymes. Using BCR-ABL1 Mbcr RGQ RT-PCR Kit allows detection and quantification of BCR-ABL1 p210 b2a2 or b3a2 and ABL1 transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.
