Description
The QIAsymphony DNA Investigator Kit enables automated purification of genomic DNA from 1-96 samples, such as swabs, filters, casework or crime-scene samples, and blood on the QIAsymphony SP. Purification is efficient, and purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis, with high signal-to-noise ratios. The QIAsymphony DNA Investigator Kit meets ISO 18385 requirements.
The QIAsymphony DNA Investigator Kit enables highly efficient purification of DNA, even from very small amounts of sample material. The combination of sample input volumes of up to 1000 µl with elution volumes as low as 30 µl enables highly sensitive detection of DNA from a wide range of sample types, including diffuse stains.
The QIAsymphony DNA Investigator Kit combines the speed and efficiency of silica-based purification of genomic and mitochondrial DNA with the convenient handling of magnetic particles (see figure "Efficient processing of magnetic particles"). Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure "Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the "Reagents and Consumables "drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control. For increased flexibility, DNA can be eluted in volumes of between 30 and 400 µl of water or Buffer ATE (modified TE buffer).
The purification procedure is designed to ensure safe and reproducible handling of precious or potentially infectious samples, and comprises 4 steps: lyse, bind, wash and elute. The user can choose different elution volumes between 30 µl to 400 µl of water or modified TE Buffer (Buffer ATE), depending on the protocol. Since the type of samples that can be processed using the QIAsymphony DNA Investigator Kit can vary greatly, there is also a variety of different pretreatments, optimized for specific sample types. Samples are lysed under denaturing conditions in the presence of proteinase K and Buffer ATL in total volumes of either 200 µl, 500 µl or 1000 µl. To meet the specific challenges of forensic samples, we have developed sample preparation protocols that ensure the highest possible first-pass success rates. Advanced protocols (ADV) use a heated binding step and increased binding time to maximize yields of pure DNA from the most difficult samples. High-Efficiency (HE) protocols address the challenge of recovering sufficient DNA from touch samples and other low-template samples. TopElute Fluid maximizes sensitivity for these difficult samples. The full range of high-efficiency forensic protocols available for different reference and casework samples is described in the table below. QIAsymphony DNA Investigator protocols Protocol Lysis volume options Enhanced binding Eluate volume TopElute Fluid Reference 200, 500 µl No 100–400 µl No Casework 200, 500, 1000 µl No 100–200 µl No Casework ADV 200, 500, 1000 µl Yes 100–200 µl No Casework HE 200, 500, 1000 µl No 30–80 µl Yes Casework HE ADV 200, 500, 1000 µl Yes 30–80 µl Yes
