Qiagen, 937266, QIAsymphony DNA Maxi Kit (96)

Name: QIAsymphony DNA Maxi Kit (96)
Brand: Qiagen
SKU: 937266

For 96 preps of 4 ml each: 2 reagent cartridges, 2 piercing lids, 2 reuse seal sets, buffers and Proteinase K solution

Catalog Number: 937266

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Category

Genomic DNA Isolation Kits

TagsDiscovery & Translational Research DNA & RNA Purification DNA Genomic DNA

Description

Automate the purification of high-quality gDNA from human whole blood samples (up to 4 ml) using the QIAsymphony SP.
Automated DNA purification from 4 ml on the QIAsymphony SP results in high yields (measured via gel electrophoresis and spectrophotometer) of high-molecular-weight DNA (see High-molecular-weight DNA) in 4.75 hours (see QIAsymphony DNA Maxi Kit workflow), and even faster for 2 ml or for fewer than 24 samples.
The QIAsymphony DNA Maxi Kit uses an interlaced system (see QIAsymphony DNA Maxi Kit workflow) that shortens the time needed to complete the process: The QIAsymphony SP takes 1 ml from the 4 ml sample and lyses that portion. While the first 1 ml is in the bind process, a second 1 ml is taken from the sample and gets lysed simultaneously. DNA from this 1 ml is bound with the same magnetic beads that were used for the first 1 ml. While this is happening, the third 1 ml is being lysed. DNA from the third 1 ml is bound in the same magnetic beads used with the previous portions. At the same time, the fourth 1 ml is being lysed. DNA from the last 1 ml portion is bound like the others.
The QIAsymphony SP performs all 4 steps (lyse, bind, wash and elute) of the purification procedure for up to 36 samples in a single run.

Specifications

General

Applications	PCR, real-time PCR, genotyping
Elution volume	200–500 µl
Main sample type	Human whole blood
Sample amount	2 or 4 ml (smaller volumes can be adjusted with PBS to 2 or 4 ml)
Yield	60–150 µg from 4 ml sample (on average; samples with particularly high or low WBC count may fall outside that range)

Ordering

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