Description
BioSprint DNA Plant Kits provide all reagents and plasticware necessary for automated purification of total DNA from plant tissue using proven magnetic-particle technology on the BioSprint 15 or BioSprint 96.
DNA can be successfully purified from a variety of plant species and material (see tables “DNA purified from a wide range of plant species using the BioSprint 96”). DNA purified from a wide range of plant species using the BioSprint 96 Sample type Amount Yield (µg) Arabidopsis, fresh leaves 50–120 mg 13–41 Cotton, fresh leaves 50 mg 10–26 Maize, fresh leaves 50 mg 10–41 Pine, fresh needles 100–190 mg 5–18 Rose, fresh new leaves 100–140 mg 40–100 Rose, fresh old leaves 100–140 mg 11–51 Soybean, fresh leaves 50 mg 39–80 Spruce, fresh needles 130–160 mg 5–13 Wheat, fresh leaves 50 mg 8–49 Maize, lyophilized leaves 5–10 mg 10–15.5 Rapeseed, lyophilized leaves 5–10 mg 13.5–23 Sunflower, lyophilized leaves 5–10 mg 57–93 Maize, dried seed powder 20–55 mg 9.8–33.5 Apple 1–6 seeds 5–30 French bean 1 bean 13–55 Grape 3 seeds 18.2–18.8 Linen 1–10 seeds 1.5–38 Lupine 2–7 seeds 18–97 Orange 1 seed 1–35 Rapeseed 1–4 seeds 8.5–47.5 Soybean 1 seed 80–105 Swedish turnip 5–50 seeds 21.5–52 Tomato 15–30 seeds 12–16 Turnip radish 4–18 seeds 11–41 Wheat 3–4 seeds 85–122 Purified DNA is up to 40 kb in size (see figure "Reproducible purification of high-quality DNA"). The DNA is ready to use in a range of downstream applications, including PCR and real-time PCR, genotyping and microsatellite analysis (see figures "High PCR performance of DNA purified using the BioSprint 15 DNA Plant Kit ” and "High PCR performance of purified DNA ”).
BioSprint DNA Plant Kits are used with the BioSprint 15 and BioSprint 96. The kits use magnetic-particle technology for rapid DNA purification from plant tissue. BioSprint instruments control an array of magnetic rods that can attract or release magnetic particles and transfer them from tube to tube or well to well. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure "Efficient processing of magnetic particles"). Since the instruments transfer magnetic particles instead of liquids, they use minimal amounts of reagents, enabling cost-efficient sample preparation.
BioSprint DNA Plant Kits enable purification of total DNA from a wide variety of plant species, including difficult plant material. Fresh, frozen or lyophilized starting material is mechanically disrupted to give a fine powder using a homogenizer such as the TissueLyser LT or TissueLyser II. The powder is resuspended in lysis buffer, thoroughly mixed and then sedimented by a short centrifugation step. Cleared plant lysates are transferred to a 5-well strip or S-Block and loaded into the instrument, which then performs all steps until pure DNA is obtained (see flowchart "BioSprint DNA Plant procedure"). The kits provide all the necessary reagents and plasticware for sample preparation, and are available in different sizes, allowing cost-efficient use of consumables.
