Description
The QIAamp DNA Blood BioRobot MDx Kit provides automated DNA purification on the BioRobot MDx workstation or the BioRobot Universal System using proven QIAamp silica-membrane technology. The fully automated procedure on the BioRobot MDx workstation requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run.
The QIAamp DNA Blood BioRobot MDx Kit process provides DNA isolation on a 96-well plate, automating processing of 32 to 96 samples (200 µl sample volume) in increments of 8 on the BioRobot MDx or the BioRobot Universal System. The fully automated procedure, including bar code reading, 20-minute load check, and complete process documentation, requires less than 2.5 hours for 96 samples, with no hands-on time. The typical yield is 3–6 µg per 200 µl healthy whole blood, with an elution volume of 200 µl. The resulting DNA performs well in downstream amplification and Southern blotting assays (see figures "No PCR inhibition "and "Reliable real-time PCR").
The QIAamp DNA Blood BioRobot MDx Kit simplifies isolation of DNA from whole blood with a fast, automated 96-well–plate procedure. No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in three efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp technology yields genomic and mitochondrial DNA from whole blood ready to use in PCR and blotting procedures. The advantages of the fully automated system include high process reliability and walkaway automation, to provide a highly efficient system for DNA purification for laboratories. QIAamp sample preparation technology is fully licensed.
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp 96-well plate. Wash buffers and ethanol are used to remove impurities, and pure, ready-to-use DNA is then eluted in water or low-salt buffer (see flowchart "protocol").
